| 1. | Cloning and expression of cdna for maize nonspecific lipid transfer protein as well as calmodulin - binding activity analysis of the expression product
一种新的烟草钙调素结合蛋白的分离鉴定 |
| 2. | However , there are many evidances provid that nonspecific lipid transfer proteins ( nsltps ) may be involved in wax transport to the surface
已有证据表明植物体内的非特异性脂转运蛋白参与了蜡质的运输。 |
| 3. | Nonspecific lipid transfer proteins ( nsltps ) are small , soluble , basic proteins that can bind and catalyze transfer of lipids in vitro , however , we do not know their functions in vivo
非特异性脂转运蛋白是一类分子量较小的可溶性碱性蛋白质，许多研究表明在体外它能结合并跨膜运输磷脂、糖脂和脂肪酸等物质。 |
| 4. | It also had common features of plant nsltps ( non - specific lipid transfer proteins ) such as eight conserved cysteine positions , high isoelectric points ( 8 . 9 ) and lack of tryptophans
其推测的氨基酸序列与genbank中注册的ltp蛋白前体的同源性达63 ，且具有植物lh蛋白共同特征，如8个保守的半肌氨酸位点，较高的等电点oi 8 |
| 5. | Reverse northern hybridization indicated that p1a5 gene was over - expressed in bract compared with that in leaf . this expression pattern was further conformed by rt - pcr . sequence analyses revealed that it is a partial sequence of a lipid transfer protein gene
通过抑制性差减杂交的到克隆pias ，反向northern杂交表明它在玖桐苞片中优势表达，即在苞片中的表达水平远远超过在处于同时期的叶片中的表达水平。 |
| 6. | The deduced amino acid sequence of p1a5 was most homologous to the lipid transfer protein 3 precursor isolated from upland cotton , the lipid transfer protein sdi - 9 isolated from common sunflower , and the nonspecific lipid - transfer protein precursor allergen pru av3 isolated from sweet cherry
对得到的该片段分析表明，它是一种脂肪转移蛋白（ ltp ）基因的部分序列。采用5 ’端race技术获得了该基因的全长cdna序列n 。 |
| 7. | This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library . the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein . the first 23 amino acids constitute the putative signal peptide , characteristic for targeting to the secretory pathway
测得th - nsltp序列全长为634bp ，含有一个非特异性脂转移蛋白与植物耐逆性的相关性研究编码112个氨基酸的阅读框架， n端的23个氨基酸组成一段信号肽序列，表明它可能和分泌有关。 |
